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What specifications are there for pipette tips
发布时间:2025-10-23 10:26:55

1. Micro pipette tip

Volume range: Usually 0.1μL - 10μL, and for some special models, it can be as low as 0.01μL.

Applicable scenarios: It is used for ultra-micro pipetting, such as enzyme and primer addition in molecular biology experiments, or sample transfer in mass spectrometry detection.

Compatible pipettes: Micropipettes with volumes ranging from 0.5 to 10μL (such as 2μL and 10μL pipettes).

Conventional pipette tip

Volume range: The mainstream is 10μL - 1000μL (1mL), and the most commonly used ones are 200μL and 1000μL.

Compatible pipettes: 20-200μL pipettes are compatible with 200μL pipettes, and 100-1000μL pipettes are compatible with 1000μL pipettes (some 500-1000μL pipettes can also be compatible with 1000μL pipettes).

Large-capacity pipette tip

Volume range: Generally 1mL - 50mL, common specifications include 5mL, 10mL, 25mL, and 50mL.

Applicable scenarios: It is used for large-scale liquid transfer, such as medium portioning, liquid transfer between centrifuge tubes, and large-volume reagent addition.

Compatible pipettes: Large-capacity pipettes with capacities of 1-5mL, 5-10mL, 10-25 ml, etc. (also known as pipette tips for pipette controllers).

Ii. Classification by pipette tip type

Different types of pipette tips are designed for specific experimental requirements, and the selection should be based on the scenario:

Universal pipette tip (non-filter element)

Features: Simple structure, no filtering device, and relatively low cost.

Applicable scenarios: General non-sensitive experiments, such as routine solution preparation and pipetting in non-sterile environments.

Filter cartridge tip (with filter cartridge)

Features: The pipette tip contains a filter membrane (usually made of polypropylene) inside, which can prevent sample aerosols from being sucked back into the pipette, avoiding cross-contamination and instrument contamination.

Applicable scenarios: Sensitive experiments, such as RNA extraction, virus detection, cell culture, PCR experiments, etc., where contamination must be strictly avoided.

Low adsorption tip

Features: The inner wall has undergone special treatment (such as siliconization and plasma treatment) to reduce the adsorption of liquids (especially nucleic acids, proteins, and organic solvents) on the inner wall of the pipette tip.

Applicable scenarios: Transfer easily adsorbed liquids, such as protein solutions, high-concentration nucleic acid solutions, ethanol and other organic solvents, to avoid sample loss.

Sterile pipette tip

Features: Sterilized (such as gamma-ray sterilization, EO sterilization), free of bacteria, nucleases and endotoxins.

Applicable scenarios: Aseptic experiments, such as cell culture, microbiological testing, clinical diagnostic sample processing, etc.


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