Regularly calibrate pipettes: Calibration is a crucial step to ensure the accuracy of pipettes. Usually, the mass of deionized water absorbed is weighed with a balance, and then its volume is calculated based on the density. Compared with the preset volume, the piston setting is adjusted if necessary for correction. It is generally recommended to conduct professional calibration every 6 to 12 months.

Select the appropriate pipette and tip: Choose the suitable type and volume range of pipette according to the experimental requirements. Single-channel pipettes are suitable for routine small-volume liquid operations, while multi-channel pipettes are ideal for handling multiple samples simultaneously. At the same time, use high-quality pipette tips that match the pipette. High-quality pipette tips can provide excellent airtight sealing, preventing leakage and bubble problems, and ensuring the accuracy of pipetting.

Correctly set the pipetting volume: When setting the pipetting volume, it should be within the rated range of the pipette. If the pipetting volume is too small, it may lead to inaccurate aspiration; if it is too large, it may cause bubbles and affect the accuracy.

Master the correct operation skills: When drawing liquid, hold the pipette vertically and fully immerse the pipette tip below the liquid column's curved surface. The immersion depth should be appropriate to avoid inhaling air or causing the liquid to stick to the outside of the pipette tip. Draw the liquid slowly to prevent the formation of bubbles. Pause for one second after aspirating the liquid and before the pipette tip leaves the liquid, and keep the pause time consistent. When discharging the liquid, gently press the piston and pause for a moment to ensure that the liquid is completely released.