The selection and compatibility of pipette tips have a crucial impact on the accuracy of pipetting, directly affecting the accuracy and repeatability of experimental results. The following is an analysis of the key influencing factors and optimization suggestions:

1.The compatibility of the pipette tip with the pipette

Tolerance matching: The dimensional tolerances of the pipette tip and the pipette cone tip must be strictly matched. Mismatch will lead to:

Leakage (insufficient negative pressure, incomplete liquid absorption).

Too tight (difficult to remove, which may cause liquid residue or volume error).

Brand compatibility: It is recommended to give priority to using the original factory pipette tips. If third-party pipette tips are used, compatibility tests (such as se

2. Tip material and design

Material influence

Polypropylene (PP) : Highly inert, suitable for biological samples, but the difference in elasticity may affect the sealing performance

Additives: Some pipette tips contain pigments or conductive fillers, which may adsorb samples (such as proteins, DNA).

Key design points

Conical design: It affects the efficiency of liquid emptying, and the low residue design can reduce errors.

Filter element: The pipette tip with a filter element can prevent aerosol contamination, but it may increase dead volume.

3. Volume range and tip selection

Capacity matching: The nominal capacity of the pipette should match the tip (for example, a 200μL pipette with a 200μL tip). Using out-of-range pipette tips may result in:

Large pipette tips for small volumes: The error rate increases (for example, 10μL of liquid evaporates and adheres to the wall significantly in a 1000μL pipette tip).

Over-capacity use of small pipette tips: Liquids may be sucked into the pipette, contaminating and damaging the equipment.