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What are the functions of pipette tips respectively?
发布时间:2025-05-22 09:48:29

1. Filter Tip

Structure: Built-in polypropylene or glass fiber filter element (pore size approximately 0.22-1 µm).

Function:

Anti-contamination: Prevent aerosol cross-contamination between the inside of the pipette and the sample (such as avoiding primer or template contamination of the pipette piston in PCR experiments).

Protect the pipette: Prevent liquid from being sucked back into the pipette to avoid corroding the piston or mechanical components.

Application scenarios:

Molecular biology (such as PCR, qPCR for anti-contamination), virus detection (such as COVID-19 nucleic acid extraction), aseptic operation (such as cell line passage).

2. Extended Tip

Structure: The length of the pipette tip tube is 30%-50% longer than that of a regular pipette tip (for example, a regular 1000 µL pipette tip is about 85 mm long, and the extended type can reach 120 mm).

Function:

Reach the bottom of the container: Suitable for suctioning liquids from deep well plates (such as 96-well / 384-well deep well plates), centrifuge tubes (such as 50 mL Falcon tubes), or the bottom of reagent bottles to avoid residual waste.

Reduce liquid adhesion to the wall: The long tube body design can reduce the contact area between the liquid and the conical part of the pipette tip during the pipetting process, lowering adsorption errors.

3. Sterilized tips (Sterile Tip)

Method of treatment: Sterilize by gamma rays, ethylene oxide (EO), or Autoclavable. Mark "Sterile" or "Autoclavable" on the package.

Function:

Avoid biological contamination: Ensure that the liquid being transferred is free of microorganisms (such as bacteria and fungi) and is suitable for experiments in a sterile environment.

Adapt to biosafety requirements: In cell therapy, gene therapy or biopharmaceuticals, prevent exogenous microorganisms from interfering with cell activity or product purity.

Application scenarios:

Primary cell culture, stem cell research, and preparation of sterile reagents (such as serum-free medium).


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