Dimensional accuracy

Compatibility with pipettes: If the inner diameter, outer diameter or length of the pipette tip is inaccurate and does not fit tightly with the pipette, leakage is likely to occur. For instance, if the inner diameter of the pipette tip is too large, the pipette cannot form a good seal. During the process of pipetting and transferring liquid, the liquid may leak from the gap between the pipette tip and the pipette, resulting in the actual amount of liquid transferred being less than the set value.

Liquid residue: Pipette tips with poor dimensional accuracy may have irregular internal shapes, which can lead to more liquid residue after pipetting. For instance, if the inner wall of the pipette tip is not smooth or has slight unevenness, the liquid will be difficult to flow out completely, thereby affecting the accuracy of the next pipetting operation. Especially when performing multiple consecutive pipetting operations, such errors will accumulate continuously.

Material uniformity

Inconsistent elasticity: Tips made of uneven materials will have different elasticity. When the pipette piston pushes the liquid in the tip, inconsistent elasticity can cause instability in the speed and volume at which the liquid is pushed out. For instance, if the material of a certain part of the pipette tip is relatively soft, when the piston is pushed, this part may deform excessively, resulting in inaccurate liquid flow.

Thermal expansion differences: Under different temperature conditions, the thermal expansion coefficients of tips made of non-uniform materials vary. If the temperature changes during the experiment, the volume of the pipette tip will change due to thermal expansion, thereby affecting the accuracy of pipetting. For instance, in some experiments that need to be conducted under different temperature conditions, such as PCR reactions, the difference in thermal expansion of pipette tip materials may lead to deviations in pipetting volume, affecting the experimental results.