Proper handling and storage of pipette tips is essential to ensure the accuracy of the experiment, avoid contamination and extend the service life of the pipette tips. The following are specific methods:

Pre-use treatment:

Check package integrity: Before use, carefully check whether the package of the suction head is intact. If the package is damaged, the suction head may be contaminated and cannot be used in experiments requiring high sterility.

Confirm the specifications and models: According to the experimental requirements and the type of pipette used, select the appropriate specifications and models of the suction head. Ensure that the suction head fits the pipette well to ensure the accuracy of the pipette.

Sterilization treatment (if necessary) : For experiments with high sterility requirements such as cell culture and molecular cloning, sterilizing suction heads are required. If the non-sterilizing suction head is purchased, it can be placed in a suitable container and sterilized by high-pressure steam sterilization (generally 121 ° C, 15-20 minutes). After sterilization, wait for the suction head to cool and dry before opening to avoid re-contamination.

Treatment during use:

Properly install the suction head: put the suction head tightly on the gun head of the pipette to ensure that the seal between the two is good to avoid air leakage affecting the pipette accuracy.

Avoid cross-contamination: When using suction heads to absorb different samples, the suction heads must be replaced to prevent cross-contamination between samples. For samples containing biohazardous substances, this principle should be strictly observed.

Prevent liquid residue: When inhaling and releasing liquid, it should be carried out in accordance with the operating specifications of the pipette, and the speed of inhaling and releasing liquid should be controlled to avoid liquid residue or hanging on the wall. For high viscosity or volatile liquids, it may be necessary to adjust the handling method appropriately.