In the fields of biological experiment, chemical analysis and pharmaceutical research and development, pipette gun is a commonly used experimental tool, its accuracy and convenience have an important impact on the reliability of experimental results. The correct installation and use of pipette nozzle is the key to ensure its accuracy. This paper will introduce in detail the installation steps of pipette nozzle, use precautions and solutions to common problems, to help experimentals better grasp the skills of pipette nozzle.

1. Installation steps of pipette gun suction head

Select the right suction head: According to the specifications of the pipette gun and experimental requirements, select the right suction head. In general, the capacity of the suction head should match the range of the pipette gun to ensure the accuracy of the pipette.

Check the quality of the suction head: Before installing the suction head, check whether the suction head is damaged, deformed or contaminated. If found, should promptly replace the new suction head.

Install the pipette: Insert the pipette vertically into the pipette, ensuring that the pipette is tightly attached to the pipette. Gently rotate the transfer gun to ensure that the suction head fits the pipette without air leakage.

Second, the use of pipette nozzle precautions

Keep the pipette vertical: When using the pipette, always keep the pipette vertical and avoid tilting or shaking to ensure the accuracy of the pipette.

Slow suction of liquid: When absorbing liquid, the button of the pipette gun should be slowly pressed to avoid rapid operation resulting in liquid splashing or air inhalation.

Avoid cross contamination: When absorbing different liquids, the suction head should be replaced in time to avoid cross contamination. At the same time, the pipette and suction head should be cleaned and disinfected regularly to ensure the accuracy and safety of the experiment.

Solutions to common problems

Suction head leakage: If the suction head leakage is found, it may be caused by the suction head and the pipette gun is not fitted or the suction head is damaged. In this case, reinstall the suction head or replace it with a new one.

Inaccurate pipette amount: Inaccurate pipette amount can be caused by improper pipette operation, mismatched suction heads, or different liquid viscosity. During operation, pay attention to keeping the pipette vertical and slowly absorbing liquid; Select the appropriate suction head; When absorbing viscous liquid, the absorption time can be appropriately increased.

Pipette blockage: Pipette blockage can be caused by impurities or sediment in the liquid blocking the suction head or the internal passage of the pipette. At this time, try to clean the suction head and the internal passage of the pipette with distilled water or ethanol; If you still can not solve the problem, you can contact professional maintenance personnel for repair.