1. Use the right suction head: To ensure better accuracy and precision, it is recommended that the pipette amount is in the range of 35%-100% of the suction head.

2. Installation of the suction head: For most brands of pipettes, especially multi-channel pipettes, it is not easy to install the suction head: in order to pursue good sealing, it is necessary to insert the pipette sleeve handle into the suction head, turn left and right or shake back and forth. Some people will use the pipette repeatedly hit the suction head to tighten, but this operation will lead to the deformation of the suction head and affect the accuracy, and serious will damage the pipette, so such an operation should be avoided.

3. Suction head immersion Angle and depth: the suction head immersion Angle is controlled within 20 degrees of inclination, and it is better to keep vertical; The recommended immersion depth of the suction head is as follows:

Pipette specification Suction depth

2µL and 10 µL 1 mm

20µL and 100 µL 2-3 mm

200µL and 1000 µL 3-6 mm

5000 µL and 10 mL 6-10 mm

For normal temperature samples, the suction head washing is helpful to improve the accuracy; However, for high temperature or low temperature samples, the suction head washing will reduce the accuracy of the operation, so please pay special attention to the user.

5. Suction speed: pipetting operation should maintain smooth and appropriate suction speed; The fast suction speed is easy to cause the sample to enter the sleeve handle, causing damage to the piston and seal ring and cross-contamination of the sample.