As a consumable used in conjunction with a pipette, tips can generally be divided into: ①. Standard tips, ②. Filter tips, ③. Low adsorption tips, ④. No heat source tips, etc.

1. Standard tips are the most widely used tips, and common tips can be used for almost all pipetting operations, which is the most economical type of tips.

2. The filter tip is a consumable designed to avoid cross-contamination, and is often used in experiments such as molecular biology, cytology, and virology.

3. For experiments with high sensitivity requirements, or precious samples or reagents that are easy to remain, you can choose low adsorption tips to improve recovery. The surface of the low adsorption tip has been hydrophobic treatment, which can reduce the low surface tension liquid leaving more residue in the tip.

PS: Wide mouth pipette, ideal for sucking sticky material, genomic DNA, cell culture medium;

Performance indicators of the tip: low adsorption, filter element, tightness, loading and ejecting force, no DNase and RNase, no pyrogen;

How to choose a suction head? "As long as the tip can fit, it is the tip that can be used"

——This is the common perception of almost all users about the adaptability of tips. This statement is partly true but not entirely true.

The tip that can be attached to the pipette can indeed form a pipetting system with the pipette to realize the pipetting function, but is this reliable? A question mark is required here. Answering this question requires data to speak for itself.

1. You may wish to test the performance after matching the pipette with the tip. After rinsing the tip, perform several repeated additions, weigh each addition, and record the reading.

2. Calculate the accuracy and precision of the pipetting operation after converting the density of the test liquid into volume.

3. What we want to choose is a tip with good accuracy. If the matching accuracy of the pipette and tip is not good, it means that the tightness of the tip and the pipette cannot be guaranteed, making the results of each operation unreproducible.

So what points should a good tip have at least? A good tip depends on concentricity, taper, and the most important point is adsorption;

1. Let’s talk about the taper first: if it is better, the match with the gun will be very good, and the suction will be more accurate;

2. Concentricity: Concentricity refers to whether the circle at the suction nozzle of the suction head and the connection between the suction head and the pipette appear to be the same center of the circle. If it is not the same center, it means that the concentricity is not good;

3. Finally, the most important one is our adsorption: the adsorption is related to the material of the tip. If the material of the tip is not good, it will affect the accuracy of pipetting, resulting in a large amount of liquid retention or referred to as For hanging on the wall, resulting in pipetting errors;

Therefore, everyone should pay special attention to the above three points when choosing a suction head

If a bad tip is placed in a row with obvious spacing, it will make a difference! You will see obvious distortions, which is an important step in choosing whether or not a good tip is good.