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What are the uses of the suction head in the laboratory?
发布时间:2026-02-25 15:18:26
The pipette tip, also known as the pipette tip in the laboratory, is the most basic and commonly used consumable material. It is used in almost all experimental procedures involving liquid sampling, addition, and transfer. Its core function is to precisely, sterilely, and without cross-contamination complete the transfer of small amounts of liquid. In routine biochemical experiments, the pipette tip is mainly used for precisely aspirating and distributing various reagents, buffers, standards, and sample solutions, ensuring that the volume of each transfer is accurate and consistent. This is the foundation for reliable experimental data. Whether it is sample preparation, standard curve creation, or reagent addition, quantitative operations cannot be completed without the use of the pipette tip. In molecular biology experiments, the use of pipettes is more frequent and crucial. For instance, in PCR system preparation, nucleic acid extraction, enzyme digestion reactions, and fluorescence quantitative PCR sample addition, sterile, enzyme-free, and DNA-free pipettes must be used to avoid external contamination that could lead to experimental failure. At the same time, by using disposable pipettes, interference between samples can be prevented, ensuring the authenticity and reliability of amplification and detection results. In cell culture-related operations, the pipette tips must meet the aseptic requirements. They are used to draw culture medium, trypsin, PBS buffer, cell suspension, etc., for cell passage, fluid replacement, inoculation, counting, etc. Aseptic pipette tips can effectively prevent bacterial, fungal, and mycoplasma contamination, ensuring the smooth progress of cell experiments. In the sample processing and detection stage, the pipette tips can be used to draw various biological samples such as serum, plasma, urine, tissue homogenate, etc., to complete sample packaging, dilution, spotting, sampling, to be added to microplates, chemiluminescent plates, test strips or detection instruments, and be compatible with the automation or manual operation of various analytical and detection equipment. For some volatile, corrosive, radioactive or toxic reagents, using pipette tips with filter elements can effectively prevent liquid backflow into the pipette, which not only protects the instrument from damage but also avoids the laboratory personnel coming into contact with dangerous substances, thereby enhancing operational safety. |