What is a pipette tip and what are its uses
发布时间:2025-08-14 14:14:29
I. Structural Features of Pipette Tips Material: Mostly made of polypropylene (PP), it features good chemical stability, resistance to most acids, alkalis and organic solvents, and is not prone to react with liquids. Some special pipette tips (such as those used in PCR experiments) will adopt low-adsorption polypropylene to reduce liquid residue. Shape: Usually conical in structure, the top is the interface for connecting to the pipette (with a suitable sealing design on the inner wall), and the bottom is the suction port (tip). The overall shape is "thick at the top and thin at the bottom", which is convenient for reaching the bottom of containers such as test tubes and microplates to draw liquid. Specification: It is divided into various models according to the volume range. Common ones include: Micro pipette tips: such as 0.1-10μL, 2-20μL, 10-100μL, etc., are suitable for handling small volumes of liquids. Conventional pipette tips: such as 100-1000μL, 500-5000μL, etc., are used for the transfer of medium-volume liquids. Large-capacity pipette tips: such as 1-10 ml, meeting the requirements of larger volume pipetting. Ii. Uses of Pipette Tips As the "execution end" of the pipette, the core function of the pipette tip is to cooperate with the pipette to achieve precise operation of liquids. It is widely used in experimental fields such as biology, chemistry, medicine, and pharmacy. Specific applications include: Precise pipetting: Accurately draw and transfer specific volumes of liquid in experiments, such as drawing standard solutions from reagent bottles or transferring sample solutions to reaction tubes, to ensure the repeatability and accuracy of the experiments. Avoid cross-contamination: As the pipette tips are for single use (or exclusively used after strict sterilization), they can prevent cross-contamination between different samples and reagents. This is particularly important in scenarios with extremely high cleanliness requirements, such as microbial culture, nucleic acid testing (such as PCR), and cell experiments. Compatible with different containers: The conical tip design of the pipette tip enables it to reach the bottom of various containers (such as centrifuge tubes, microplates, and small-bore reagent bottles), ensuring that the liquid is fully absorbed and reducing residue. Special experimental requirements: Some dedicated pipette tips can meet specific experimental requirements, such as: Filter core pipette tip: Built-in filter membrane to prevent liquid backflow and contamination of the pipette interior; Low adsorption tip: Reduces the residue of liquid on the inner wall of the tip and improves the pipetting accuracy of trace liquids. Sterilized tips: Sterilized by gamma rays and other methods, suitable for aseptic experiments (such as cell culture, vaccine preparation). |