What conditions should a good pipette tip meet?
发布时间:2025-07-29 11:18:44
Pipette tips are the core consumables of pipettes, and their quality directly affects the accuracy, repeatability and experimental safety of pipetting. A high-quality pipette tip needs to meet the following key conditions I. Physical Structure and Compatibility Precise dimensional tolerance The connection part between the pipette tip and the pipette tip (such as the conical interface) must be strictly matched to ensure a tight seal and avoid air leakage during pipetting (air leakage will cause the pipetting volume to be smaller and the error to increase). The tip of the pipette has a uniform pore size, free of burrs, deformations or cracks, to prevent liquid residue, dripping or sticking to the wall during suction (especially for micro pipette tips, the tip precision requirement is even higher for those under 10μL). Wide adaptability It is compatible with mainstream brand pipettes (such as Eppendorf, Gilson, Thermo, etc.), eliminating the need for separate purchases due to brand restrictions and reducing experimental costs. Ii. Material and Chemical Compatibility High-quality aw materials Medical-grade polypropylene (PP) is usually adopted and has the following characteristics: Strong chemical inertness: Resistant to acids, alkalis, and organic solvents (such as ethanol, methanol, DMSO, etc.), it does not react with common experimental reagents, thus avoiding sample contamination. High-temperature resistance: It can withstand 121℃ high-pressure steam sterilization (moist heat sterilization), and does not deform or release harmful substances after sterilization (suitable for sterile scenarios such as cell culture and microbiological experiments). High transparency: It is convenient to observe the liquid level and bubbles inside the pipette tip (especially when transferring colored or turbid liquids, it can reduce operational errors). During the production process, no plasticizers, lubricants or other impurities are added to avoid interfering with experiments (for example, in PCR experiments, there must be no RNase, DNase or pyrogens, otherwise nucleic acids will be degraded or cytotoxicity will be caused). |